BACKGROUND Interferon gamma, IFN-γ, is a major immune-modulating molecule produced mainly by T-cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFN-γ receptors and respond to IFN-γ-induced signaling by up-regulating MHC class I expression. Human IFN-γ does not show cross-reactivity with mouse.
Recombinant human IFN-γ is a non-glycosylated protein, containing 144 amino acids and having a molecular mass of 16.88 kDa.
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RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
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